Bovine Alkaline Phosphatase ELISA kit

 E11A0031 Bovine alp activity kit

 

For the detection of samples from bovine species, use an ELISA kit for bovine alkaline phosphatase. Alkaline Phosphatase can also be called AKP, ALKP, Basic Phosphatase.

 

Principle of the Assay

 

alkaline phosphatase detection kit applies the competitive enzyme immunoassay technique utilizing an anti-ALP antibody and an ALP-HRP conjugate. The assay sample and buffer are incubated together with ALP-HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for the HRP enzyme. The product of the enzyme-substrate reaction forms a blue-colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of the color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALP concentration since ALP from samples and ALP-HRP conjugate compete for the anti-ALP antibody binding site. Since the number of sites is limited, as more sites are occupied by ALP from the sample, fewer sites are left to bind ALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALP concentration in each sample is interpolated from this standard curve.

 

Quality Control on Bovine Alkaline Phosphatase ELISA kit

Coefficient of Variance

Intra Variation% ＜10%

Inter Variation% ＜12%

Recovery

92-103%

Linearity

Diluent Ratio

Range %

1:2

90-105

1:4

88-108

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ALP and analogues was observed.

 

 

For more information about alkaline phosphatase colorimetric assay, please feel free to contact us!

 

As a professional drug residue test kit factory, Bluegene has high-quality mouse igg elisa kits for sale. Welcome to contact us if needed.

 


Comments

Post a Comment

Popular posts from this blog

Fish ELISA Kits

Image
  Fish are often used in environmental monitoring, embryo research, tumor research and others. Among them, zebrafish is the most commonly used. The zebrafish is a common tropical fish, which is native to South Asia. The zebrafish have a slender body with an adult length of 3~4cm, and they do not require high water quality. About 3 months after hatching, the fish reach sexual maturity, and mature fish can lay eggs every few days. The eggs are fertilized in vitro and develop in vitro. The embryo development is synchronous and fast, and the embryo body is transparent.   Fish ELISA Kits  Types Fish Cyclic Adenosine Monophosphate ELISA kit (E17C0027)   Cat. No.: E17C0027   Detection Range: 50-1000pmol/ml   Reactivity: Fish   Sensitivity: 1.0pmol/ml   Fish Pyruvate Kinase A ELISA kit (E17P0666)   Cat. No.: E17P0666   Detection Range: 1.0-25ng/ml   Reactivity: Fish   Sensitivity: 0.1ng/ml   Fish Testosterone ELISA kit (E17T0007) ...
Read more

Hamster ELISA Kits

Image
  A hamster is also known as suslik, which is an animal domesticated by wild animals and entered the laboratory. There are 8 kinds of hamsters for experimental research. There are mainly two kinds of hamsters as experimental animals: Golden hamster, Mesometritus auratus and Chinese hamster, Crucetulus griseus. Among them, the Chinese hamsters are widely used in nutrition and infectious disease research. In addition, the Chinese hamsters are often used for the study of diabetes, and they are good animal models of true diabetes.   Hamster ELISA Kits  Types Hamster Total Adiponectin ELISA kit (E18A0125)   Cat. No.: E18A0125   Detection Range: 1.0-25ng/ml   Reactivity: Hamster   Sensitivity: 0.1ng/mL       Hamster   ELISA  kit (E18I0004)   Cat. No.: E18I0004   Detection Range: 0.5-10ng/ml   Reactivity: Hamster   Sensitivity: 0.1ng/ml   Hamster Leptin ELISA kit (E18L0038)   Cat. No.: E18L0038   Det...
Read more

BlueGene Biotech HH-H0019-1 HEK 293 Host Cell Protein ELISA Kit, G2-96T

Image
  BlueGene Biotech HH-H0019-1 HEK293 Host Cell Protein A ssay K it , G2-96T HEK 293 Host Cell Protein ELISA Kit, G2 is for research use only, it should not be used in clinical diagnostic procedures. The kit is intended for the quantitative determination of residual host cell protein in Cell Culture Supernatants, Protein Purification Process, and End-Product expressed by HEK 293 expression systems.   Specifications Of HH-H0019-1 HEK293 HCP ELISA  Kit, G2     Principle of the Assay   This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrat...
Read more