Bovine Acetyl Coenzyme A Carboxylase ELISA kit
E11A0913 Bovine Acetyl Coenzyme A Carboxylase ELISA kit Bovine acetyl co a carboxylase ELISA kit is suitable for the detection of samples from Bovine species. Acetyl Coenzyme A Carboxylase can also be called ACC. Principle of the Assay A competitive enzyme immunoassay approach is used in the ACC ELISA kit along with an anti-ACC antibody and an ACC-HRP conjugate. For one hour, the ACC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the ACC concentration since ACC from sam...