Bovine Total Adiponectin ELISA kit

 E11A0125 Bovine Total Adiponectin ELISA kit

 

Bovine Total Adiponectin ELISA kit is suitable for the detection of samples from Bovine species. Total Adiponectin can also be called as 30 kDa adipocyte complement related protein; 30 kDa adipocyte complement-related protein; ACDC; ACRP 30; ACRP30; ADIPO; Adipocyte; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP 30, ACRP30, ADIPO, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, AdipoQ, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, APM 1, apM-1, ApM1, Gelatin-binding protein.

 

Principle of the Assay

 

ADPN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADPN antibody and an ADPN-HRP conjugate. The assay sample and buffer are incubated together with ADPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ADPN antibody binding site between ADPN from samples and ADPN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ADPN. As more sites are taken up by ADPN from the sample, fewer sites are left that can bind ADPN-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. This standard curve is interpolated to determine the ADPN concentration in each sample.

 

Quality Control on Bovine Total Adiponectin ELISA kit

Coefficient of Variance

Intra Variation% ＜10%

Inter Variation% ＜12%

Recovery

95-106%

Linearity

Diluent Ratio

Range %

1:2

93-103

1:4

89-108

1:8

86-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ADPN and analogues was observed.

 

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