Canine Galectin 3 ELISA kit

 E08G0052 Canine Galectin 3 ELISA Kit

 

Canine Galectin 3 ELISA kit is suitable for the detection of samples from Canine species. Galectin 3 can also be called LGALS3, CBP35, GALBP, GALIG, MAC2, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, Laminin-binding protein, Lectin L-29, GAL3.

 

GAL3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GAL3 antibody and an GAL3-HRP conjugate. The assay sample and buffer are incubated together with GAL3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAL3 concentration since GAL3 from samples and GAL3-HRP conjugate compete for the anti-GAL3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GAL3 from the sample, fewer sites are left to bind GAL3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL3 concentration in each sample is interpolated from this standard curve.

 

Quality Control on Canine Galectin 3 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	91-105%
Linearity	Diluent Ratio	Range %
	1:2	87-108
	1:4	86-111
	1:8	83-113
Specificity/Cross-reactivity	No significant cross-reactivity or interference between GAL3 and analogues was observed.

 

 

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